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COVID-19 Human IgM IgG ELISA kit (Spike Protein)

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​96T　￥150,000

​（tax excluded）

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Manual

​SDS

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Specification

Measurement method:　　　Indirect ELISA

Measurement wavelength:　　　450 nm

Measurement sample:　　　Serum

Species reactivity:　　　　Human

Storage temperature:　　　Keep HRP-conjugated anti-human IgM/IgG antibody at or below -20 ° C. 

                                     Keep other reagents and immobilized plate at 2-8 ° C

Expiration date:　　　This product is valid for 5 months after production.

Immobilized antige:　　　SARS-CoV-2 spike protein (S1+S2 domain, mammalian expression system)

Kit contents

1. SARS-CoV-2 spike protein-immobilized plate

2. HRP-conjugated anti-human IgM antibody

3. HRP-conjugated anti-human IgG antibody

4. Washing buffer（10× PBST）

5. Non-fat milk (for blocking buffer)

6. Diluent buffer (10×)

7. R-1: Chromogenic substrate (TMB)

8. R-2: Stop reagent (1 mol/L hydrochloric acid)

9. Microplate seal

×2

4 μL×1

4 μL×1

50 mL×2

×1

50 mL×1

10 mL×2

10 mL×2

×2

Measurement principle

  1. The immobilized SARS-CoV-2 recombinant spike protein on a plate is reacted with the antibody (hereinafter referred to as anti-spike protein antibody) in a sample that recognizes it.

  2.  After the reaction, the sample is removed by washing.

  3.  React anti-spike protein antibody in the plate with HRP (horseradish peroxidase)- conjugated anti-human  IgM / IgG antibodies.

  4. Remove excess HRP-conjugated anti-human IgM / IgG antibodies by washing. Add a chromogenic substrate and measure the absorbance.

EN_測定図(Spike).png

Significance of measurement

This kit is a research ELISA kit to detect human IgM and IgG antibodies that recognize the spike protein of SARS-CoV-2, the causative virus of Covid-19.

Virus detection can be broadly divided into two approaches: one is to detect the virus itself, such as PCR method, and the other is to detect the immune response that develops during infection (IgG, IgM, IgA). In common infections, IgM is an antibody produced early in the infection and disappears with relief of symptoms. IgG begins to be produced later than IgM and continues to be produced for months after healing. This is known to reduce the symptoms of the next infection or to prevent the onset of the next infection

Publications using this product

      J Med Virol. 2021 Feb 23. PubMed PMID: 33620098.

Related literature

​​​

      Pediatrics. 2020 Dec. PubMed PMID: 32879033

      Nat Commun. 2020 Nov 27. PubMed PMID: 33247152

      PLoS One. 2020 Nov 23. PubMed PMID: 33227020

      Sci Immunol. 2020 Oct 8. PubMed PMID: 33033173​

  • Anges Yadouleton et al.
    Limited Specificity of Serologic Tests for SARS-CoV-2 Antibody Detection, Benin

      Emerg Infect Dis. 2020 Dec 1. PubMed PMID: 33261717

  • Ricardo Savirón-Cornudella et al.
    Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) universal screening in gravids during labor and delivery

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  • Emily Garnett et al.
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  • Lenin Mahimainathan et al.
    Evaluation of SARS-CoV-2 Serological Testing in Patients with Multiple Myeloma and Other Hematologic Malignancies on Monoclonal Antibody Therapies

      Diagnostics (Basel). 2020 Nov 24. PubMed PMID: 33255154

  • Harvey W Kaufman et al.
    Insights from Patterns of SARS-CoV-2 Immunoglobulin G Serology Test Results from a National Clinical Laboratory, United States, March-July 2020

      Popul Health Manag. 2020 Nov 19. PubMed PMID: 33216694

  • Martin Risch et al.
    Temporal Course of SARS-CoV-2 Antibody Positivity in Patients with COVID-19 following the First Clinical Presentation

      Biomed Res Int. 2020. PubMed PMID: 33224987

  • Ainhoa Gutiérrez-Cobos et al.
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      Eur J Clin Microbiol Infect Dis. 2020 Nov 24. PubMed PMID: 33236268

  • Adrian M Shields et al.
    Serological responses to SARS-CoV-2 following non-hospitalised infection: clinical and ethnodemographic features associated with the magnitude of the antibody response

      medRxiv. Preprint. 2020 Nov 16. PubMed PMID: 33236029

  • Constant Gillot et al.
    An Original ELISA-Based Multiplex Method for the Simultaneous Detection of 5 SARS-CoV-2 IgG Antibodies Directed against Different Antigens

      J Clin Med. 2020 Nov 21. PubMed PMID: 33233405

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    High prevalence of SARS-CoV-2 antibodies in care homes affected by COVID-19: Prospective cohort study, England

      EClinicalMedicine. 2020 Nov 6. PubMed PMID: 33173854

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    Development and evaluation of a serological test for diagnosis of COVID-19 with selected recombinant spike proteins

      Eur J Clin Microbiol Infect Dis. 2020 Nov 12. PubMed PMID: 33184753

  • Melkon G DomBourian et al.
    Analysis of COVID-19 convalescent plasma for SARS-CoV-2 IgG using two commercial immunoassays

      J Immunol Methods. 2020 Nov. PubMed PMID: 32828791

  • Ling Li et al.
    Characteristics and serological patterns of COVID-19 convalescent plasma donors: optimal donors and timing of donation

      Transfusion. 2020 Aug. PubMed PMID: 32627216

  • Guillaume Beaudoin-Bussières et al.
    Decline of Humoral Responses against SARS-CoV-2 Spike in Convalescent Individuals

      mBio. 2020 Oct 16. PubMed PMID: 33067385

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    Persistence and decay of human antibody responses to the receptor binding domain of SARS-CoV-2 spike protein in COVID-19 patients

       Sci Immunol. 2020 Oct 8. PubMed PMID: 33033172

  • Phuong Nguyen-Contant et al.
    S Protein-Reactive IgG and Memory B Cell Production after Human SARS-CoV-2 Infection Includes Broad Reactivity to the S2 Subunit

       mBio. 2020 Sep 25. PubMed PMID: 32978311

  • James R. Byrnes et al.
    Competitive SARS-CoV-2 Serology Reveals Most Antibodies Targeting the Spike Receptor-Binding Domain Compete for ACE2 Binding

       mSphere. 2020 Sep 16. PubMed PMID: 32938700

  • Mohammad Hosein Taziki Balajelini et al.
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  • Tyler J Ripperger et al.
    Orthogonal SARS-CoV-2 Serological Assays Enable Surveillance of Low-Prevalence Communities and Reveal Durable Humoral Immunity

       Immunity. 2020 Oct 14. PubMed PMID: 33129373

  • Pedro Martínez-Fleta et al.
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       J Immunol. 2020 Nov 4. PubMed PMID: 33148714

  • Eshan U Patel et al.
    Comparative performance of five commercially available serologic assays to detect antibodies to SARS-CoV-2 and identify individuals with high neutralizing titers

       J Clin Microbiol. 2020 Nov 2. PubMed PMID: 33139419

  • Zahra Rikhtegaran Tehrani et al.
    Performance of nucleocapsid and spike-based SARS-CoV-2 serologic assays

       PLoS One. 2020 Nov 2. PubMed PMID: 33137138

  • Meret Huber et al.
    Postinfectious Onset of Myasthenia Gravis in a COVID-19 Patient

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  • Ruta Kulkarni et al.
    Anti-SARS-CoV-2 IgG antibody response among Indian COVID-19 patients using β-propiolactone-inactivated, whole virus-based indirect ELISA

       J Virol Methods. 2021 Jan. PubMed PMID: 33126149

  • Bernd Jahrsdörfer et al.
    Independent side-by-side validation and comparison of four serological platforms for SARS-CoV-2 antibody testing

       J Infect Dis. 2020 Oct 16. PubMed PMID: 33064789

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       Eur J Clin Microbiol Infect Dis. 2020 Oct 20. PubMed PMID: 33078222

  • Xiaohui Wang et al.
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       mBio . 2020 Oct 20. PubMed PMID: 33082264

  • Alessandra Sperotto et al.
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       J Med Virol. 2020 Oct 8. PubMed PMID: 33030745

  • Suliman A Alharbi et al.
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       Cureus. 2020 Sep 6. PubMed PMID: 33047077

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    Diagnostic performance of commercially available COVID-19 serology tests in Brazil

       Int J Infect Dis. 2020 Oct 9. PubMed PMID: 33039612

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  • Álvaro A Faccini-Martínez et al.
    Serological cross-reactivity using a SARS-CoV-2 ELISA test in acute Zika virus infection, Colombia

       Int J Infect Dis. 2020 Sep 28. PubMed PMID: 33002616

  • Nguyen N Nguyen et al.
    Correlation of ELISA method with three other automated serological tests for the detection of anti-SARS-CoV-2 antibodies

       PLoS One. 2020 Oct 6. PubMed PMID: 33022019

  • Massimo Guerriero et al.
    Prevalence of asymptomatic SARS-CoV-2-positive individuals in the general population of northern Italy and evaluation of a diagnostic serological ELISA test: a cross-sectional study protocol

       BMJ Open. 2020 Oct 6. PubMed PMID: 33028562

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       Eur J Microbiol Immunol (Bp). 2020 Sep 25. PubMed PMID: 32979256

  • Elitza S Theel et al.
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  • Mei San Tang et al.
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  • Pauline H Herroelen et al.
    Humoral Immune Response to SARS-CoV-2

       Am J Clin Pathol. 2020 Aug 18. PubMed PMID: 32808976

  • Anna E Merrill et al.
    Head-to-Head Comparison of Two SARS-CoV-2 Serology Assays

       J Appl Lab Med. 2020 Jul 27. PubMed PMID: 32717056

  • Marzia Nuccetelli et al.
    Combined anti-SARS-CoV-2 IgA, IgG, and IgM Detection as a Better Strategy to Prevent Second Infection Spreading Waves

       Immunol Invest. 2020 Sep 18. PubMed PMID: 32945214

  • Reuben McGregor et al.
    Collaborative networks enable the rapid establishment of serological assays for SARS-CoV-2 during nationwide lockdown in New Zealand

      PeerJ . 2020 Sep 3. PubMed PMID: 32953275

  • Suzanne Pickering et al.
    Comparative assessment of multiple COVID-19 serological technologies supports continued evaluation of point-of-care lateral flow assays in hospital and community healthcare settings

      PLoS Pathog . 2020 Sep 24. PubMed PMID: 32970782

  • Gang Xu et al.
    Evaluation of Orthogonal Testing Algorithm for Detection of SARS-CoV-2 IgG Antibodies

      Clin Chem. 2020 Sep 7. PubMed PMID: 32894753

  • Diego O. Andrey et al.
    Head-to-Head Accuracy Comparison of Three Commercial COVID-19 IgM/IgG Serology Rapid Tests

      J Clin Med. 2020 Aug. PubMed PMID: 32722191

  • Diego O. Andrey et al.
    Sensitivity, Specificity and Predictive Values of Molecular and Serological Tests for COVID-19: A Longitudinal Study in Emergency Room

      Eur J Clin Invest. 2020 Jul 21. PubMed PMID: 32691863

  • Zeno Bisoffi et al.
    Diagnostic accuracy of Augurix COVID‐19 IgG serology rapid test

      Diagnostics (Basel). 2020 Sep 3. PubMed PMID: 32899333

  • Eshan Patel et al.
    Comparative performance of five commercially available serologic assays to detect antibodies to SARS-CoV-2 and identify individuals with high neutralizing titers

      medRxiv . 2020 Sep 2. PubMed PMID: 32908987

  • Georg M. N. Behrens et al.
    Strategic Anti-SARS-CoV-2 Serology Testing in a Low Prevalence Setting: The COVID-19 Contact (CoCo) Study in Healthcare Professionals

      Infect Dis Ther. 2020 Sep 4. PubMed PMID: 32886335

  • Oktavija Đaković Rode et al.
    Antibody response and the clinical presentation of patients with COVID-19 in Croatia: the importance of a two-step testing approach

      Eur J Clin Microbiol Infect Dis. 2020 Sep 4. PubMed PMID: 32886230

  • Clarence W Chan et al.
    Analytical and Clinical Evaluation of the Automated Elecsys Anti–SARS-CoV-2 Antibody Assay on the Roche cobas e602 Analyzer

      Am J Clin Pathol. 2020 Aug 20. PubMed PMID: 32814955

  • Fleur Wolff et al.
    Monitoring antibody response following SARS-CoV-2 infection: diagnostic efficiency of 4 automated immunoassays

      Diagn Microbiol Infect Dis. 2020 Nov. PubMed PMID: 32829098

  • Niko Kohmer et al.
    Brief clinical evaluation of six high-throughput SARS-CoV-2 IgG antibody assays

      J Clin Virol. 2020 Aug. PubMed PMID: 32505777

  • Christian Wechselberger et al.
    Performance evaluation of serological assays to determine the immunoglobulin status in SARS-CoV-2 infected patients

      J Clin Virol. 2020 Oct. PubMed PMID: 32810840

  • Hui-Xia Gao et al.
    Detection of serum immunoglobulin M and immunoglobulin G antibodies in 2019 novel coronavirus infected patients from different stages

      Chin Med J (Engl). 2020 Jun 20. PubMed PMID: 32221133

  • Sonny Elizaldi et al.
    SARS-CoV-2 infection induces robust germinal center CD4 T follicular helper cell responses in rhesus macaques

      Res Sq. 2020 Aug 14. PubMed PMID: 32818217

  • Jeffrey D Whitman et al.
    Evaluation of SARS-CoV-2 serology assays reveals a range of test performance

      Nat Biotechnol. 2020 Aug 27. PubMed PMID: 32855547

  • Verena Haselmann et al.
    Results of the first pilot external quality assessment (EQA) scheme for anti-SARS-CoV2-antibody testing

      Clin Chem Lab Med . 2020 Aug 27. PubMed PMID: 32853163

  • Maria Infantino et al.
    Closing the Serological Gap in the Diagnostic Testing for COVID‐19: The Value of Anti‐SARS‐CoV‐2 IgA Antibodies

      J Med Virol. 2020 Aug 13. PubMed PMID: 32790181

  • Verena Haselmann et al.
    Comparison of test performance of commercial anti-SARS-CoV-2 immunoassays in serum and plasma samples

      Clin Chim Acta. 2020 Nov. PubMed PMID: 32652161

  • G. Caruana et al.
    Diagnostic strategies for SARS-CoV-2 infection and interpretation of microbiological results​

      Clin Microbiol Infect. 2020 Sep. PubMed PMID: 32593741

  • David Peterhoff et al.
    A highly specific and sensitive serological assay detects SARS-CoV-2 antibody levels in COVID-19 patients that correlate with neutralization

      Infection. 2020 Aug 21. PubMed PMID: 32827125

  • Kasopefoluwa Y Oguntuyo et al.
    Quantifying absolute neutralization titers against SARS-CoV-2 by a standardized virus neutralization assay allows for cross-cohort comparisons of COVID-19 sera

      medRxiv. 2020 Aug 15. PubMed PMID: 32817961

  • Kathleen M McAndrews et al.
    Heterogeneous antibodies against SARS-CoV-2 spike receptor binding domain and nucleocapsid with implications on COVID-19 immunity

      JCI Insight. 2020 Aug 14. PubMed PMID: 32796155

  • Aaron J Siegler et al.
    Protocol for a national probability survey using home specimen collection methods to assess prevalence and incidence of SARS-CoV-2 infection and antibody response

      Ann Epidemiol. 2020 Aug 10. PubMed PMID: 32791199

  • Zahra Rikhtegaran Tehrani et al.
    Specificity and Performance of Nucleocapsid and Spike-based SARS-CoV-2 Serologic Assays

      medRxiv. 2020 Aug 7. PubMed PMID: 32793933

  • Sebastian Hörber et al.
    Evaluation of three fully-automated SARS-CoV-2 antibody assays

      Clin Chem Lab Med. 2020 Aug 4. PubMed PMID: 32745068

  • Felipe Pérez-García et al.
    Alltest rapid lateral flow immunoassays is reliable in diagnosing SARS-CoV-2 infection from 14 days after symptom onset: A prospective single-center study

      J Clin Virol. 2020 Aug. PubMed PMID: 32504945

  • Anita S. Iyer et al.
    Dynamics and significance of the antibody response to SARS-CoV-2 infection

      medRxiv. Preprint. 2020 Jul 20. PubMed PMID: 32743600

  • Giorgio Caturegli et al.
    Clinical Validity of Serum Antibodies to SARS-CoV-2

      Ann Intern Med. 2020 Jul 6. PubMed PMID: 32628534

  • Jitendra Prasad Mathuria et al.
    Laboratory diagnosis of SARS-CoV-2 - A review of current methods 

      J Infect Public Health. 2020 Jul. PubMed PMID: 32534946

  • Alexander Krüttgen et al.
    Comparison of four new commercial serologic assays for determination of SARS-CoV-2 IgG

      J Clin Virol. 2020 Jul. PubMed PMID: 32416599

  • Fatima Amanat et al.

       A serological assay to detect SARS-CoV-2 seroconversion in humans

      Nat Med. 2020 Jul. PubMed PMID: 32398876

  • Mayara Lisboa Bastos et al.
    Diagnostic Accuracy of Serological Tests for covid-19: Systematic Review and Meta-Analysis

      BMJ. 2020 Jul. PubMed PMID: 32611558

  • Lisa Sophie Pflüger et al.
    Clinical evaluation of five different automated SARS-CoV-2 serology assays in a cohort of hospitalized COVID-19 patients

      J Clin Virol. 2020 Jul 17. PubMed PMID: 32763809

  • Corine H. GeurtsvanKessel et al.
    An evaluation of COVID-19 serological assays informs future diagnostics and exposure assessment

      Nat Commun. 2020 Jul 6. PubMed PMID: 32632160

  • Daniel Shyu et al.
    Laboratory Tests for COVID-19: A Review of Peer-Reviewed Publications and Implications for Clinical UIse

      Mo Med. 2020 May-Jun. PubMed PMID: 32636542

  • Patrick Sean Sullivan et al.
    Detection of SARS-CoV-2 RNA and Antibodies in Diverse Samples: Protocol to Validate the Sufficiency of Provider-Observed, Home-Collected Blood, Saliva, and Oropharyngeal Samples

      JMIR Public Health Surveill. 2020 Apr-Jun. PubMed PMID: 32310815

  • Thomas Nicol et al.
    Assessment of SARS-CoV-2 Serological Tests for the Diagnosis of COVID-19 Through the Evaluation of Three Immunoassays: Two Automated Immunoassays (Euroimmun and Abbott) and One Rapid Lateral Flow Immunoassay (NG Biotech)

      J Clin Virol. 2020 Jun 15. PubMed PMID: 32593133

  • Benjamin Meyer et al​.
    Validation of a commercially available SARS-CoV-2 serological immunoassay

      Clin Microbiol Infect. 2020 Jun 27. PubMed PMID: 3260380

  • Silvia Stringhini et al.
    Seroprevalence of anti-SARS-CoV-2 IgG antibodies in Geneva, Switzerland (SEROCoV-POP): a population-based study

      Lancet. 2020 Jun 11. PubMed PMID: 32534626

  • Antonio Vittorino Gaddi et al.
    The Strategic Alliance between Clinical and Molecular Science in the War against SARS-CoV-2, with the Rapid-Diagnostics Test as an Indispensable Weapon for Front Line Doctors

      Int J Mol Sci. 2020 Jun. PubMed PMID: 32580529

  • Kathleen G. Beavis et al.

       Evaluation of the EUROIMMUN Anti-SARS-CoV-2 ELISA Assay for detection of IgA and IgG antibodies

      J Clin Virol. 2020 Aug. PubMed PMID: 32485620

  • AJ Jääskeläinen et al.
    Performance of six SARS-CoV-2 immunoassays in comparison with microneutralization

      J Clin Virol. 2020 Aug. PubMed PMID: 32563180

  • Baoqing Sun et al.
    Kinetics of SARS-CoV-2 Specific IgM and IgG Responses in COVID-19 Patients

      Emerg Microbes Infect. 2020 Dec. PubMed PMID: 32357808

  • Feng-Cai Zhu et al.
    Safety, tolerability, and immunogenicity of a recombinant adenovirus type-5 vectored COVID-19 vaccine: a dose-escalation, open-label, non-randomised, first-in-human trial

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  • Andrea P Espejo et al.

       Review of Current Advances in Serologic Testing for COVID-19

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       Antibody tests for identification of current and past infection with SARS-CoV-2

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       coronaviruses SARS-CoV and MERS-CoV

       J Clin Virol. 2020 Jun 22. PubMed PMID: 32623350

  • Wan Ni Chia et al.
    Serological Differentiation Between COVID-19 and SARS Infections

      Emerg Microbes Infect. 2020 Jun 12. PubMed PMID: 32529906

  • Lakshmanane Premkumar et al.
    The receptor binding domain of the viral spike protein is an immunodominant and highly specific target of antibodies in SARS-CoV-2 patients

      Sci Immunol. 2020 Jun 11. PubMed PMID: 32527802

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    Seroprevalence of Immunoglobulin M and G Antibodies Against SARS-CoV-2 in China

      Nat Med. 2020 Jun 5. PubMed PMID: 32504052​

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    SARS‐CoV‐2 Seroconversion in Humans: A Detailed Protocol for a Serological Assay, Antigen Production, and Test Setup

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    A Method to Prevent SARS-CoV-2 IgM False Positives in Gold Immunochromatography and Enzyme-Linked Immunosorbent Assays

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    Serum IgA, IgM, and IgG responses in COVID-19

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  • Wanbing Liu et al.
    Evaluation of Nucleocapsid and Spike Protein-Based Enzyme-Linked Immunosorbent Assays for Detecting Antibodies Against SARS-CoV-2

      J Clin Microbiol . 2020 May 26. PubMed PMID: 32229605​

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    Detection of serum IgM and IgG for COVID-19 diagnosis

      Sci China Life Sci. 2020 May. PubMed PMID: 32270436

  • Alba Grifoni et al.
    Targets of T Cell Responses to SARS-CoV-2 Coronavirus in Humans with COVID-19 Disease and Unexposed Individuals

      Cell. 2020 May 20. PubMed PMID: 32473127

  • Trevor R F Smith et al.
    Immunogenicity of a DNA Vaccine Candidate for COVID-19
    Nat Commun. 2020 May 20. PubMed PMID: 32433465

  • Marie Tré-Hardy et al.
    The role of serology for COVID-19 control: Population, kinetics and test performance do matter

     J Infect. 2020 May 15. PubMed PMID: 32417311

  • Johannes Korth et al.
    SARS-CoV-2-specific antibody detection in healthcare workers in Germany with direct contact to COVID-19 patient

      J Clin Virol. 2020 Jul (Published online 2020 May 13.) PubMed PMID: 32434708​

  • Fatima Amanat et al.
    A Serological Assay to Detect SARS-CoV-2 Seroconversion in Humans
    Nat Med. 2020 May 12. PubMed PMID: 32511441

  • Jiajia Xie et al.
    Characteristics of patients with coronavirus disease (COVID‐19) confirmed using an IgM‐IgG antibody test
    J Med Virol. 2020 May 7. PubMed PMID: 32330303

  • Anne J Jääskeläinen et al.
    Evaluation of commercial and automated SARS-CoV-2 IgG and IgA ELISAs using coronavirus disease (COVID-19) patient samples

      Euro Surveill. 2020 May 7. PubMed PMID: 32400364

  • Rongqing Zhao et al.
    Early Detection of SARS-CoV-2 Antibodies in COVID-19 Patients as a Serologic Marker of Infection
    Clin Infect Dis. 2020 May. PubMed PMID: 32357209

  • Hongzhou Lu et al.
    An Evolving Approach to the Laboratory Assessment of COVID-19
    J Med Virol . 2020 Apr 29. PubMed PMID: 32347966

  • Quan-Xin Long et al.
    Antibody Responses to SARS-CoV-2 in Patients With COVID-19
    Nat Med. 2020 Apr 29. PubMed PMID: 32350462

  • Kai Duan et al.
    Effectiveness of convalescent plasma therapy in severe COVID-19 patients

      Proc Natl Acad Sci U S A. 2020 Apr 28. PubMed PMID: 32253318

  • Juanjuan Zhao et al.
    Antibody Responses to SARS-CoV-2 in Patients of Novel Coronavirus Disease 2019
    Cin Infect Dis. 2020 Mar 28. PubMed PMID: 32221519

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