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COVID-19 Human IgM IgG ELISA kit (Spike Protein)

96T ¥150,000

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Manual

​SDS

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Specification

Measurement method:   Indirect ELISA

Measurement wavelength:   450 nm

Measurement sample:   Serum

Species reactivity:    Human

Storage temperature:   Keep HRP-conjugated anti-human IgM/IgG antibody at or below -20 ° C. 

                                     Keep other reagents and immobilized plate at 2-8 ° C

Expiration date:   This product is valid for 5 months after production.

Immobilized antige:   SARS-CoV-2 spike protein (S1+S2 domain, mammalian expression system)

Kit contents

1. SARS-CoV-2 spike protein-immobilized plate

2. HRP-conjugated anti-human IgM antibody

3. HRP-conjugated anti-human IgG antibody

4. Washing buffer(10× PBST)

5. Non-fat milk (for blocking buffer)

6. Diluent buffer (10×)

7. R-1: Chromogenic substrate (TMB)

8. R-2: Stop reagent (1 mol/L hydrochloric acid)

9. Microplate seal

×2

4 μL×1

4 μL×1

50 mL×2

×1

50 mL×1

10 mL×2

10 mL×2

×2

Measurement principle

  1. The immobilized SARS-CoV-2 recombinant spike protein on a plate is reacted with the antibody (hereinafter referred to as anti-spike protein antibody) in a sample that recognizes it.

  2.  After the reaction, the sample is removed by washing.

  3.  React anti-spike protein antibody in the plate with HRP (horseradish peroxidase)- conjugated anti-human  IgM / IgG antibodies.

  4. Remove excess HRP-conjugated anti-human IgM / IgG antibodies by washing. Add a chromogenic substrate and measure the absorbance.

EN_測定図(Spike).png

Significance of measurement

This kit is a research ELISA kit to detect human IgM and IgG antibodies that recognize the spike protein of SARS-CoV-2, the causative virus of Covid-19.

Virus detection can be broadly divided into two approaches: one is to detect the virus itself, such as PCR method, and the other is to detect the immune response that develops during infection (IgG, IgM, IgA). In common infections, IgM is an antibody produced early in the infection and disappears with relief of symptoms. IgG begins to be produced later than IgM and continues to be produced for months after healing. This is known to reduce the symptoms of the next infection or to prevent the onset of the next infection

Publications using this product

      J Med Virol. 2021 Feb 23. PubMed PMID: 33620098.

Related literature

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  • Aaron J Siegler et al.
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  • Zahra Rikhtegaran Tehrani et al.
    Specificity and Performance of Nucleocapsid and Spike-based SARS-CoV-2 Serologic Assays

      medRxiv. 2020 Aug 7. PubMed PMID: 32793933

  • Sebastian Hörber et al.
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  • Anita S. Iyer et al.
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      medRxiv. Preprint. 2020 Jul 20. PubMed PMID: 32743600

  • Giorgio Caturegli et al.
    Clinical Validity of Serum Antibodies to SARS-CoV-2

      Ann Intern Med. 2020 Jul 6. PubMed PMID: 32628534

  • Jitendra Prasad Mathuria et al.
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      J Infect Public Health. 2020 Jul. PubMed PMID: 32534946

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      J Clin Virol. 2020 Jul. PubMed PMID: 32416599

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      Nat Med. 2020 Jul. PubMed PMID: 32398876

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      BMJ. 2020 Jul. PubMed PMID: 32611558

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      J Clin Virol. 2020 Jul 17. PubMed PMID: 32763809

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      J Clin Virol. 2020 Jun 15. PubMed PMID: 32593133

  • Benjamin Meyer et al​.
    Validation of a commercially available SARS-CoV-2 serological immunoassay

      Clin Microbiol Infect. 2020 Jun 27. PubMed PMID: 3260380

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    Seroprevalence of anti-SARS-CoV-2 IgG antibodies in Geneva, Switzerland (SEROCoV-POP): a population-based study

      Lancet. 2020 Jun 11. PubMed PMID: 32534626

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      Int J Mol Sci. 2020 Jun. PubMed PMID: 32580529

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       Evaluation of the EUROIMMUN Anti-SARS-CoV-2 ELISA Assay for detection of IgA and IgG antibodies

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    Performance of six SARS-CoV-2 immunoassays in comparison with microneutralization

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    Characteristics of patients with coronavirus disease (COVID‐19) confirmed using an IgM‐IgG antibody test
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    Evaluation of commercial and automated SARS-CoV-2 IgG and IgA ELISAs using coronavirus disease (COVID-19) patient samples

      Euro Surveill. 2020 May 7. PubMed PMID: 32400364

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