COVID-19 Human IgM IgG ELISA kit(Nucleocapsid Protein)
Measurement method: Indirect ELISA
Measurement wavelength: 450 nm
Measurement sample: Serum
Species reactivity: Human
Storage temperature: Store at 2-8 ° C
Expiration date: This product is valid for 12 months after production.
Immobilized antigen: SARS-CoV-2 nucleocapsid protein (Full length, E. coli expression system)
1. SARS-CoV-2 nucleocapsid protein-immobilized plate
2. HRP-conjugated anti-human IgM antibody
3. HRP-conjugated anti-human IgG antibody
4. Wash buffer(10× PBST)
5. Bovine serum albumin (for diluent)
6. Diluent buffer (10× )
7. R-1: Chromogenic substrate (TMB)
8. R-2: Stop reagent (1 mol/L hydrochloric acid)
9. Microplate seal
The SARS-CoV-2 recombinant nucleocapsid protein on an immobilized plate is reacted with an antibody (hereinafter referred to as human IgM/IgG) in a sample that recognizes it.
After the reaction, the sample is removed by washing.
React with human IgM/IgG on immobilized plates with HRP (horseradish peroxidase)- conjugated anti-human IgM/IgG antibodies.
Remove excess HRP-conjugated anti-human IgM/IgG antibodies by washing. Add a chromogenic substrate and measure the absorbance.
Significance of measurement
This kit is a research ELISA kit to detect human IgM and IgG antibodies that recognize the
nucleocapsid protein of SARS-CoV-2, the causative virus of Covid-19.
Virus detection can be broadly divided into two approaches: one is to detect the virus itself,
such as PCR method, and the other is to detect the immune response that develops during infection (IgG, IgM, IgA). In common infections, IgM is an antibody produced early in the infection and disappears with relief of symptoms. IgG begins to be produced later than IgM and continues to be produced for months after healing. This is known to reduce the symptoms of the next infection or to prevent the onset of the next infection.
Xin Xu et al.
Seroprevalence of Immunoglobulin M and G Antibodies Against SARS-CoV-2 in China
Nat Med. 2020 Jun 5. PubMed PMID: 32504052
Wanbing Liu et al.
Evaluation of Nucleocapsid and Spike Protein-Based Enzyme-Linked Immunosorbent Assays for Detecting Antibodies Against SARS-CoV-2
J Clin Microbiol . 2020 May 26. PubMed PMID: 32229605
Fatima Amanat et al.
A Serological Assay to Detect SARS-CoV-2 Seroconversion in Humans
Nat Med. 2020 May 12. PubMed PMID: 32511441
Nandini Sethuraman et al.
Interpreting Diagnostic Tests for SARS-CoV-2
JAMA. 2020;323(22):2249-2251. PubMed PMID: 32374370
Fei Xiang et al.
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Clin Infect Dis. 2020 Apr 19. PubMed PMID: 32306047
Sarah Ee Fang Yong et al.
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Lancet Infect Dis. 2020 Apr 21. PubMed PMID: 32330439
Juanjuan Zhao et al.
Antibody Responses to SARS-CoV-2 in Patients of Novel Coronavirus Disease 2019
Cin Infect Dis. 2020 Mar 28. PubMed PMID: 32221519
Li Guo et al.
Profiling Early Humoral Response to Diagnose Novel Coronavirus Disease (COVID-19)
Clin Infect Dis. 2020 Mar 21. PubMed PMID: 32198501
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