COVID-19 Human IgM IgG ELISA kit(Nucleocapsid Protein)

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Specification

Measurement method:   Indirect ELISA

Measurement wavelength:   450 nm

Measurement sample:   Serum

Species reactivity:    Human

Storage temperature:   Store at 2-8 ° C

Expiration date:   This product is valid for 12 months after production.

Immobilized antigen: SARS-CoV-2 nucleocapsid protein (Full length, E. coli expression system)

Kit contents

1. SARS-CoV-2 nucleocapsid protein-immobilized plate

2. HRP-conjugated anti-human IgM antibody

3. HRP-conjugated anti-human IgG antibody

4. Wash buffer(10× PBST)

5. Bovine serum albumin (for diluent)

6. Diluent buffer (10× )

7. R-1: Chromogenic substrate (TMB)

8. R-2: Stop reagent (1 mol/L hydrochloric acid)

9. Microplate seal

×2

20 μL×1

20 μL×1

100 mL×1

×1

50 mL×1

22 mL×1

22 mL×1

×2

Measurement principle

  1. The SARS-CoV-2 recombinant nucleocapsid protein on an immobilized plate is reacted with an antibody (hereinafter referred to as human IgM/IgG) in a sample that recognizes it.

  2. After the reaction, the sample is removed by washing.

  3. React with human IgM/IgG on immobilized plates with HRP (horseradish peroxidase)- conjugated anti-human IgM/IgG antibodies.

  4. Remove excess HRP-conjugated anti-human IgM/IgG antibodies by washing. Add a chromogenic substrate and measure the absorbance.

Significance of measurement

This kit is a research ELISA kit to detect human IgM and IgG antibodies that recognize the

nucleocapsid protein of SARS-CoV-2, the causative virus of Covid-19.
Virus detection can be broadly divided into two approaches: one is to detect the virus itself,

such as PCR method, and the other is to detect the immune response that develops during infection (IgG, IgM, IgA). In common infections, IgM is an antibody produced early in the infection and disappears with relief of symptoms. IgG begins to be produced later than IgM and continues to be produced for months after healing. This is known to reduce the symptoms of the next infection or to prevent the onset of the next infection.

Related literature

      Clin Infect Dis. 2020 Oct 3. PubMed PMID: 33011792

      Version 1. bioRxiv. 2020 Mar. PubMed PMID: 32511324

      J Clin Virol. 2020 Nov. PubMed PMID: 32927357

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      Emerg Infect Dis. 2020 Dec 1. PubMed PMID: 33261717

      Rev Esp Quimioter. 2020 Sep 18. PubMed PMID: 32945157

      Biomed Res Int. 2020. PubMed PMID: 33224987

      Eur J Clin Microbiol Infect Dis. 2020 Nov 24. PubMed PMID: 33236268

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      J Clin Med. 2020 Nov 21. PubMed PMID: 33233405

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       J Clin Microbiol. 2020 Nov 2. PubMed PMID: 33139419

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       J Infect Dis. 2020 Oct 16. PubMed PMID: 33064789

       mBio . 2020 Oct 20. PubMed PMID: 33082264

       Diseases. 2020 Sep 23. PubMed PMID: 32977485

       Int J Infect Dis. 2020 Oct 9. PubMed PMID: 33039612

       Int J Infect Dis. 2020 Sep 28. PubMed PMID: 33002616

       Front Cell Infect Microbiol. 2020 Sep 4. PubMed PMID: 33014893

       PLoS One. 2020 Oct 6. PubMed PMID: 33022019

       J Clin Microbiol. 2020 Jul 23. PubMed PMID: 32513859

       Am J Clin Pathol. 2020 Aug 18. PubMed PMID: 32808976

       J Appl Lab Med. 2020 Jul 27. PubMed PMID: 32717056

       Immunol Invest. 2020 Sep 18. PubMed PMID: 32945214

      PLoS Pathog . 2020 Sep 24. PubMed PMID: 32970782

      Diagnostics (Basel). 2020 Sep 3. PubMed PMID: 32899333

      medRxiv . 2020 Sep 2. PubMed PMID: 32908987

      Infect Dis Ther. 2020 Sep 4. PubMed PMID: 32886335

      J Clin Virol. 2020 Aug. PubMed PMID: 32505777

      Chin Med J (Engl). 2020 Jun 20. PubMed PMID: 32221133

      Res Sq. 2020 Aug 14. PubMed PMID: 32818217

      Nat Biotechnol. 2020 Aug 27. PubMed PMID: 32855547

      Trials. 2020 Jun 3. PubMed PMID: 32493494

      EJ Med Virol. 2020 Jul 27. PubMed PMID: 32667733

      Emerg Microbes Infect. 2020 Dec. PubMed PMID: 32357808

      JCI Insight. 2020 Aug 14. PubMed PMID: 32796155

      medRxiv. 2020 Aug 7. PubMed PMID: 32793933

      J Infect Public Health. 2020 Jul. PubMed PMID: 32534946

      J Clin Virol. 2020 Jul. PubMed PMID: 32416599

      BMJ. 2020 Jul. PubMed PMID: 32611558

      Microbes Infect. 2020 May-June. PubMed PMID: 32425648​

      JMIR Public Health Surveill. 2020 Apr-Jun. PubMed PMID: 32310815

      Mo Med. 2020 May-Jun. PubMed PMID: 32636542

  • Jonathan J Deeks et al.

       Antibody tests for identification of current and past infection with SARS-CoV-2

       Cochrane Database Syst Rev. 2020 Jun 25. PubMed PMID: 32584464

  • Andrea P Espejo et al.

       Review of Current Advances in Serologic Testing for COVID-19

       Am J Clin Pathol. 2020 Jun 25. PubMed PMID: 32583852

  •   Ahidjo Ayouba et al.

       Multiplex detection and dynamics of IgG antibodies to SARS-CoV2 and the highly pathogenic human

       coronaviruses SARS-CoV and MERS-CoV

  J Clin Virol. 2020 Jun 22. PubMed PMID: 32623350

      Int J Mol Sci. 2020 Jun. PubMed PMID: 32580529

      Lancet. 2020 13-19 June. PubMed PMID: 32450106

      Emerg Microbes Infect. 2020 Jun 12. PubMed PMID: 32529906

      Clin Chim Acta. 2020 Oct. PubMed PMID: 32526218

      Nat Med. 2020 Jun 5. PubMed PMID: 32504052​​​

      Science. 2020 Jun 5. PubMed PMID: 32414781

      Cell Mol Immunol. 2020 May 28. PubMed PMID: 32467617

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References

1.)Keiichi Hiramatsu, Standard Textbook of standard microbiology, 11th edition, Medical study (2012)

2.)Anu Haveri , “Serological and molecular findings during SARS-CoV-2 infection: the first case study in Finland,” January to February 2020 , Eurosurveillance.  Volume 25, Issue 11, 19, Mar 2020.

3.)Wanbing Liu, “Evaluation of Nucleocapsid and Spike Protein-based ELISAs for detecting antibodies against SARS-CoV-2”, J Clin Microbiol. 2020 Mar 30.

4.)Li Guo, “Profiling Early Humoral Response to Diagnose Novel Coronavirus Disease (COVID-19)”, Clin Infect Dis. 2020 Mar 21.

5.)Juanjuan Zhao, “Antibody responses to SARS-CoV-2 in patients of novel coronavirus disease 2019”, Clin Infect Dis. 2020 Mar 21.

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