COVID-19 Human IgA ELISA kit (Spike Protein)(Full-Length)

96T ¥150,000

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Product Specifications

Measurement method : Indirect ELISA
Measurement wavelength : 450 nm
Measurement sample : Serum, saliva, sputum
Species reactivity : Human
Storage temperature : Store at 2-8° C. 
Expiration date : 6 months after production
Immobilized antigen : SARS-CoV-2 Spike protein (S1 + S2 domain, mammalian expression system)

Kit contents

1. SARS-CoV-2 spike protein-immobilized plate

2. HRP-conjugated anti-human IgA antibody

3. Washing buffer(10× PBST)

4. Non-fat milk (for blocking buffer)

5. Diluent buffer (10×)

6. R-1: Chromogenic substrate (TMB)

7. R-2: Stop reagent (1 mol/L hydrochloric acid)

8. Microplate seal

×1

10 μL×1

50 mL×2

×1

50 mL×1

10 mL×2

10 mL×2

×2

Measurement principle

  1. The SARS-CoV-2 recombinant spike protein immobilized on the plate is recognized and reacted with the antibody (hereinafter, abbreviated as anti-spike protein antibody) in a sample

  2.  After the reaction, the sample is removed by washing.

  3.  The anti-spike protein antibody is reacted with HRP (Horseradish peroxidase)-conjugated anti-human IgA antibody.

  4. Remove excess HRP-conjugated anti-human IgA antibody by washing. Add a chromogenic substrate and measure the absorbance.

Significance of measurement

This kit is a research ELISA kit to detect human IgA antibody that recognizes the spike protein of SARS-CoV-2, the causative virus of Covid-19.
Virus detection can be broadly divided into two approaches: one is to detect the virus itself, such as PCR method, and the other is to detect the immune response that develops during infection (IgG, IgM, IgA). IgA is particularly abundant in mucous membranes such as saliva and the nasopharynx, and generally has the function of infection prevention.

Related literature

       Clin Chim Acta. 2020 Sep 28. PubMed PMID: 33002475

       BMJ Open. 2020 Oct 6. PubMed PMID: 33028562

       PLoS One. 2020 Oct 9. PubMed PMID: 33035234

       Immunol Invest. 2020 Sep 18. PubMed PMID: 32945214

      PLoS Pathog . 2020 Sep 24. PubMed PMID: 32970782

      J Clin Virol. 2020 Oct. PubMed PMID: 32810840​​

      medRxiv. 2020 Aug 7. PubMed PMID: 32793933

  • Fatima Amanat et al.

       A serological assay to detect SARS-CoV-2 seroconversion in humans

       Nat Med. 2020 Jul. PubMed PMID: 32398876

      Cochrane Database Syst Rev. 2020 Jun 25. PubMed PMID: 32584464

      Sci Immunol. 2020 Jun 11. PubMed PMID: 32527802​​

  • Nadin Younes et al.
    Challenges in Laboratory Diagnosis of the Novel Coronavirus SARS-CoV-2

     Viruses. 2020 May 26. PubMed PMID: 32466458​​

References

1.)Keiichi Hiramatsu, Standard Textbook of standard microbiology, 11th edition, Medical study (2012)

2.)Andrea Padoan, IgA-Ab response to spike glycoprotein of SARS-CoV-2 in patients with COVID-19: A longitudinal study, Clinica Chimica Acta, 507, 164-166, Aug., 2020

5.)Yin Xia Chao, The role of IgA in COVID-19, Brain, Behavior, and Immunity, 87, Jul., 2020 

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