COVID-19 Human IgA ELISA kit (Nucleocapsid Protein)
Measurement method : Indirect ELISA
Measurement wavelength : 450 nm
Measurement sample : Serum, saliva, sputum
Species reactivity : Human
Storage temperature : Store at 2-8° C.
Expiration date : 12 months after production
Immobilized antigen : SARS-CoV-2 Nucleocapsid protein (Full length, E. coli expression system)
1. SARS-CoV-2 nucleocapsid protein-immobilized plate
2. HRP-conjugated anti-human IgA antibody
3. Washing buffer（10× PBST）
4. Bovine serum albumin (for diluent buffer)
5. Diluent buffer (10×)
6. R-1: Chromogenic substrate (TMB)
7. R-2: Stop reagent (1 mol/L hydrochloric acid)
8. Microplate seal
The SARS-CoV-2 recombinant nucleocapsid protein immobilized on the plate is recognized and reacted with the antibody (hereinafter, abbreviated as anti-nucleocapsid protein antibody) in a sample.
After the reaction, the sample is removed by washing
The anti-spike protein antibody is reacted with HRP (Horseradish peroxidase)-conjugated anti-human IgA antibody
Remove excess HRP-conjugated anti-human IgA antibody by washing. Add a chromogenic substrate and measure the absorbance.
Significance of measurement
This kit is a research ELISA kit to detect human IgA antibody that recognizes the nucleocapsid protein of SARS-CoV-2, the causative virus of Covid-19.
Virus detection can be broadly divided into two approaches: one is to detect the virus itself, such as PCR method, and the other is to detect the immune response that develops during infection (IgG, IgM, IgA). IgA is particularly abundant in mucous membranes such as saliva and the nasopharynx, and generally has the function of infection prevention.
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Antibody tests for identification of current and past infection with SARS-CoV-2
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Challenges in Laboratory Diagnosis of the Novel Coronavirus SARS-CoV-2
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2.)Andrea Padoan, IgA-Ab response to spike glycoprotein of SARS-CoV-2 in patients with COVID-19: A longitudinal study, Clinica Chimica Acta, 507, 164-166, Aug., 2020
3.)Yin Xia Chao, The role of IgA in COVID-19, Brain, Behavior, and Immunity, 87, Jul., 2020