Research use only products (RUO)
E. coli measurement kit
Measurement method: Enzyme activity assay
Excitation wavelength: 360 nm
Measurement wavelength: 460 nm
Samples: Sewage, industrial wastewater, river water, groundwater, drinking water
Species reactivity: E.coli
Storage temperature: Store at 2-8 ℃
Expiration date: 6 months after production
Measuring equipment: Fluorescence microplate reader
The plate with E. coli selective freeze-dried medium 96 well × 4
・Measure 96 samples at the same time
・Measurement time is only 3 hours
・Reasonable price for 250 JPY per test
・Only 200 μL of sample volume is needed
(1) Set the plate reader at 37 °C
(2) Make holes in the seal attached to the plate (or remove the seal.)
(3) Dispense 200μL of the test sample into each well of medium-containing 96-well plate.
(4) Set the excitation wavelength at 360 nm and the measurement wavelength at 460 nm. Measure every 10 minutes for 3 hrs.
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1.) Hisashi Satoh, Kai Kikuchi, Yutaka Katayose, Shu Tsuda, Reiko Hirano, Yuga Hirakata, Masaaki Kitajima, Satoshi Ishii, Mamoru Oshiki, Masashi Hatamoto, Masahiro Takahashi, Satoshi Okabe (2020) Simple and Reliable Enumeration of Escherichia coli Concentrations in Wastewater Samples by Measuring β-D-glucuronidase (GUS) Activities via a Microplate Reader. Science of The Total Environment. In press
2.) Hisashi SATOH, Shu TSUDA Shu, Kai KIKUCHI Kai, Reiko HIRANO Reiko (2019) “Development of a simple and rapid method for determining coliforms in wastewater based on fluorogenic enzymatic substrate” Journal of Japan Sewage Works Association. 56(684), 110-117.