Research use only products (RUO)
E. coli measurement kit
Measurement method: Enzyme activity assay
Excitation wavelength: 360 nm
Measurement wavelength: 460 nm
Samples: Sewage, industrial wastewater, river water, groundwater, drinking water
Species reactivity: E.coli
Storage temperature: Store at 2-8 ℃
Expiration date: 6 months after production
Measuring equipment: Fluorescence microplate reader
The plate with E. coli selective freeze-dried medium 96 well × 4
・Measure 96 samples at the same time
・Measurement time is only 3 hours
・Reasonable price for 250 JPY per test
・Only 200 μL of sample volume is needed
(1) Set the plate reader at 37 °C
(2) Make holes in the seal attached to the plate (or remove the seal.)
(3) Dispense 200μL of the test sample into each well of medium-containing 96-well plate.
(4) Set the excitation wavelength at 360 nm and the measurement wavelength at 460 nm. Measure every 10 minutes for 3 hrs.
J.-B. Burnet 2019.
“Autonomous online measurement of beta-D-glucuronidase activity in surface water: is it suitable for rapid E. coli monitoring?”
Water Research. Vol.152 pp.241-250.
F.J. Ferrero 2016.
“A Novel Handheld Fluorimeter for Rapid Detection of Escherichia coli in Drinking Water.”
IEEE Sensors Journal. Vol.16, pp.5136–5144.
I. George 2000.
“Use of enzymatic methods for rapid enumeration of coliforms in freshwaters.”
Journal of Applied Microbiology. Vol.88, pp.404–413.
N.S.K. Gunda 2016.
“A hydrogel based rapid test method for detection of Escherichia coli (E. coli) in contaminated water samples.”
Analyst. Vol.141, pp.2920–2929.
B. Heery 2016.
“ColiSense, today’s sample today: A rapid on-site detection of β-D-Glucuronidase activity in surface water as a surrogate for E. coli.”
Talanta. Vol.148, pp.75–83.
N. Hesari 2016.
“A biosensor platform for rapid detection of E. coli in drinking water.”
Enzyme and Microbial Technology. Vol.83, pp.22–28
G. Magro 2014.
“Synthesis and Application of Resorufin β-D-Glucuronide, a Low-Cost Chromogenic Substrate for Detecting Escherichia coli in Drinking Water.”
Environmental Science & Technology. Vol.48, pp.9624–9631.
D. Wildeboer 2010.
“Rapid detection of Escherichia coli in water using a hand-held fluorescence detector.”
Water Research. Vol.44, pp.2621–2628.
Hisashi Satoh, Kai Kikuchi, Yutaka Katayose, Shu Tsuda, Reiko Hirano, Yuga Hirakata, Masaaki Kitajima, Satoshi Ishii, Mamoru Oshiki, Masashi Hatamoto, Masahiro Takahashi, Satoshi Okabe (2020) Simple and Reliable Enumeration of Escherichia coli Concentrations in Wastewater Samples by Measuring β-D-glucuronidase (GUS) Activities via a Microplate Reader. Science of The Total Environment. In press
Hisashi SATOH, Shu TSUDA Shu, Kai KIKUCHI Kai, Reiko HIRANO Reiko (2019) “Development of a simple and rapid method for determining coliforms in wastewater based on fluorogenic enzymatic substrate” Journal of Japan Sewage Works Association. 56(684), 110-117.