COVID-19 Human IgA ELISA kit (Spike Protein)(S1)

96T ¥150,000

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Product Specifications

Measurement method : Indirect ELISA
Measurement wavelength : 450 nm
Measurement sample : Serum, saliva, sputum
Species reactivity : Human
Storage temperature : Store at 2-8° C. 
Expiration date : 12 months after production
Immobilized antigen : SARS-CoV-2 Spike protein (S1 domain, E. coli expression system)

Kit contents

1. SARS-CoV-2 spike protein(S1)-immobilized plate

2. HRP-conjugated anti-human IgA antibody

3. Washing buffer(10× PBST)

4. Bovine serum albumin (for diluent buffer)

5. Diluent buffer (10×)

6. R-1: Chromogenic substrate (TMB)

7. R-2: Stop reagent (1 mol/L hydrochloric acid)

8. Microplate seal


10 μL×1

100 mL×1


50 mL×1

22 mL×1

22 mL×1


Measurement principle

  1. The SARS-CoV-2 recombinant spike protein (S1) immobilized on the plate is recognized and reacted with the antibody (hereinafter, abbreviated as anti-spike protein antibody) in a sample.

  2.  After the reaction, the sample is removed by washing.

  3.  The anti-spike protein antibody is reacted with HRP (Horseradish peroxidase)-conjugated anti-human IgA antibody

  4. Remove excess HRP-conjugated anti-human IgA antibody by washing. Add a chromogenic substrate and measure the absorbance.

Significance of measurement

This kit is a research ELISA kit to detect human IgA antibody that recognizes the spike protein of SARS-CoV-2, the causative virus of Covid-19.
Virus detection can be broadly divided into two approaches: one is to detect the virus itself, such as PCR method, and the other is to detect the immune response that develops during infection (IgG, IgM, IgA). IgA is particularly abundant in mucous membranes such as saliva and the nasopharynx, and generally has the function of infection prevention.

Related literature

       J Infect Dis. 2020 Oct 16. PubMed PMID: 33064789

       Eur J Clin Microbiol Infect Dis. 2020 Oct 20. PubMed PMID: 33078222

       Cureus. 2020 Sep 6. PubMed PMID: 33047077

       Int J Infect Dis. 2020 Oct 9. PubMed PMID: 33039612

       Am J Clin Pathol. 2020 Aug 18. PubMed PMID: 32808976

      Infect Dis Ther. 2020 Sep 4. PubMed PMID: 32886335

      Am J Clin Pathol. 2020 Aug 20. PubMed PMID: 32814955

      Diagn Microbiol Infect Dis. 2020 Nov. PubMed PMID: 32829098

      Res Sq. 2020 Aug 14. PubMed PMID: 32818217

      Clin Chem Lab Med . 2020 Aug 27. PubMed PMID: 32853163

      J Med Virol. 2020 Aug 13. PubMed PMID: 32790181

  • Felipe Pérez-García et al.
    Alltest rapid lateral flow immunoassays is reliable in diagnosing SARS-CoV-2 infection from 14 days after symptom onset: A prospective single-center study

      J Clin Virol. 2020 Aug. PubMed PMID: 32504945

  • Andrea Padoan et al.
    IgA-Ab Response to Spike Glycoprotein of SARS-CoV-2 in Patients With COVID-19: A Longitudinal Study

      Clin Chim Acta. 2020 Aug. PubMed PMID: 32343948

  • Kathleen G. Beavis et al.

       Evaluation of the EUROIMMUN Anti-SARS-CoV-2 ELISA Assay for detection of IgA and IgG antibodies

      J Clin Virol. 2020 Aug. PubMed PMID: 32485620

  • AJ Jääskeläinen et al.
    Performance of six SARS-CoV-2 immunoassays in comparison with microneutralization

      J Clin Virol. 2020 Aug. PubMed PMID: 32563180

      Infection. 2020 Aug 21. PubMed PMID: 32827125

      medRxiv. 2020 Aug 7. PubMed PMID: 32793933

      J Infect Public Health. 2020 Jul. PubMed PMID: 32534946

  • Fatima Amanat et al.

       A serological assay to detect SARS-CoV-2 seroconversion in humans

      Nat Med. 2020 Jul. PubMed PMID: 32398876

      BMJ. 2020 Jul. PubMed PMID: 32611558

      J Med Virol. 2020 Jul 27. PubMed PMID: 32667733

      medRxiv. Preprint. 2020 Jul 20. PubMed PMID: 32743600

      J Clin Virol. 2020 Jul 17. PubMed PMID: 32763809

      Nat Commun. 2020 Jul 6. PubMed PMID: 32632160

      JMIR Public Health Surveill. 2020 Apr-Jun. PubMed PMID: 32310815

      Clin Microbiol Infect. 2020 Jun 27. PubMed PMID: 32603801

      Cochrane Database Syst Rev. 2020 Jun 25. PubMed PMID: 32584464

      J Clin Virol. 2020 Jun 15. PubMed PMID: 32593133

      Emerg Microbes Infect. 2020 Jun 12. PubMed PMID: 32529906

  • Roshan J. D'Cruz et al.
    Laboratory Testing Methods for Novel Severe Acute Respiratory Syndrome-Coronavirus-2 (SARS-CoV-2)

      Front Cell Dev Biol. 2020 Jun 4. PubMed PMID: 32582718

  • J Van Elslande et al.
    Diagnostic Performance of Seven Rapid IgG/IgM Antibody Tests and the Euroimmun IgA/IgG ELISA in COVID-19 Patients

      Clin Microbiol Infect. 2020 May. PubMed PMID: 32473953

  • Huan Ma et al.
    Serum IgA, IgM, and IgG responses in COVID-19

      Cell Mol Immunol. 2020 May 28. PubMed PMID: 32467617​​

  • Nadin Younes et al.
    Challenges in Laboratory Diagnosis of the Novel Coronavirus SARS-CoV-2

     Viruses. 2020 May 26. PubMed PMID: 32466458

  • Alba Grifoni et al.
    Targets of T Cell Responses to SARS-CoV-2 Coronavirus in Humans with COVID-19 Disease and Unexposed Individuals

      Cell. 2020 May 20. PubMed PMID: 32473127​

  • Marie Tré-Hardy et al.
    The role of serology for COVID-19 control: Population, kinetics and test performance do matter

     J Infect. 2020 May 15. PubMed PMID: 32417311

      Euro Surveill. 2020 May 7. PubMed PMID: 32400364

      Emerg Infect Dis. 2020 Apr 8. PubMed PMID: 32267220


1.)Keiichi Hiramatsu, Standard Textbook of standard microbiology, 11th edition, Medical study (2012)

2.)Andrea Padoan, IgA-Ab response to spike glycoprotein of SARS-CoV-2 in patients with COVID-19: A longitudinal study, Clinica Chimica Acta, 507, 164-166, Aug., 2020

3.)Yin Xia Chao, The role of IgA in COVID-19, Brain, Behavior, and Immunity, 87, Jul., 2020 

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